ECL Western Blotting Substrate Kit ab65623 from Abcam

Product Specs
ItemECL Western Blotting Substrate Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65623
Quantity50 TEST, 500 TEST
ApplicationsWestern blot
Detection TargetECL

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) PEPITEM Treatment Ameliorates EAE in Mice by Reducing CNS Inflammation, Leukocyte Infiltration, Demyelination, and Proinflammatory Cytokine ProductionInt J Mol SciDecember 8, 2023Mohammed Alassiri, Fahd Al Sufiani, Mohammed Aljohi, Asma Alanazi, Aiman Saud Alhazmi, Bahauddeen M. Alrfaei, Hasan Alnakhli, Yasser A. Alshawakir, Saleh M. Alharby, Abdullah Y. Almubarak, et al.washing four times (5 min each), it was incubated in an ECL Western blotting substrate kit (Catalog No ab65623) from Abcam (Cambridge, United Kingdom) for 2 min. Each band on the membranes was detected using a ChemiDoc

(2) Celecoxib/Cyclodextrin Eye Drop Microsuspensions: Evaluation of In Vitro Cytotoxicity and Anti-VEGF Efficacy for Retinal DiseasesPharmaceuticsNovember 28, 2023Phatsawee Jansook, Hay Man Saung Hnin Soe, Rathapon Asasutjarit, Theingi Tun, Hay Marn Hnin, Phyo Darli Maw, Tanapong Watchararot, Thorsteinn LoftssonProtein bands were visualized after extensive washing with enhanced chemiluminescence substrate (cat. no. ab65623; Abcam, Cambridge, UK) using the ChemiDoc Imaging System (ChemiDocTM, Bio-Rad Laboratories Inc., Benicia,

(3) Prunetin in a GPR30-dependent manner mitigates renal ischemia/reperfusion injury in rats via interrupting indoxyl sulfate/TLR4/TRIF, RIPK1/RIPK3/MLKL, and RIPK3/PGAM5/DRP-1 crosstalkSaudi Pharmaceutical JournalNovember 1, 2023Ahmed B. Hamed, Hanan S. El-Abhar, Dalaal M. Abdallah, Kawkab A. Ahmed, Yasmin S. AbulfadlHRP-labeled secondary antibody (# ab97057; Abcam) after rinsing with TBST 5 times. The ECL detection kit (# ab65623; Abcam) was used to visualize the expressed proteins of interest, and the intensity of the bands was

(4) METTL3 promotes proliferation and migration of colorectal cancer cells by increasing SNHG1 stabilityMolecular Medicine ReportsSeptember 26, 2023Yeqiu Xu, Yuxin Bao, Guanzhen Qiu, Huinan Ye, Ming He, Xilin Weiab205719; Abcam) were added at room temperature for 1 h. The ECL Western Blotting substrate kit (cat. no. ab65623; Abcam) was utilized to visualise the target proteins and ImageJ software (v2; National Institutes of

(5) Hexavalent Chromium Inhibited Zebrafish Embryo Development by Altering Apoptosis- and Antioxidant-Related GenesCurr Issues Mol BiolAugust 18, 2023Khoa Dang Dang, Chi Nguyen Quynh Ho, Huy Duc Van, Son Thanh Dinh, Quynh Thi Truc Nguyen, Tram Thi Thuy Nguyen, Xuyen Thi Ngoc Kien, Tuyet Van Dao, Hung Viet Nong, Minh Thai Nguyen, et al.primary antibodies, respectively. The blots were visualized using the ECL Western Blotting Substrate Kit (ab65623, Abcam, Cambridge, MA, USA). Imaging was carried out with an X-ray film. The quantification of the exposed

(6) Acetaminophen impairs ferroptosis in the hippocampus of septic mice by regulating glutathione peroxidase 4 and ferroptosis suppressor protein 1 pathwaysBrain and BehaviorJuly 13, 2023Jing Chu, Hong Li, Zhihao Yuan, Wenyu Zhou, Yang Yu, Yonghao YuNitrocellulose membranes were rinsed with TBST and treated with an enhanced chemiluminescence assay kit (Cat#ab65623, Abcam). The density of the strips was scanned, photographed, and analyzed using a Bio‐Rad Image Analysis

(7) Muscle-derived stem cell exosomes with overexpressed miR-214 promote the regeneration and repair of rat sciatic nerve after crush injury to activate the JAK2/STAT3 pathway by targeting PTENFrontiers in Molecular NeuroscienceMay 26, 2023Xiangyu Zeng, Wei Bian, Ziwen Liu, Jianming Li, Shuai Ren, Jian Zhang, Haoran Zhang, Bu Tegeleqi, Guanyi He, Mingyan Guan, et al.incubation at room temperature. Protein bands were detected using the ECL Western Blotting Substrate Kit (ab65623, Abcam, United Kingdom) and analyzed for protein levels using Image-Pro Plus software. Statistical

(8) N(6)‑methyladenosine‑induced long non‑coding RNA PVT1 regulates the miR‑27b‑3p/BLM axis to promote prostate cancer progressionInternational journal of oncologyJanuary 1, 2023Chen B, Liu C, Long H, Bai G, Zhu Y, Xu H.temperature before the visualization of protein bands using an ECL Western Blotting Substrate kit (cat. no. ab65623, Abcam). Immunohistochemical staining Paraffin-embedded tissues sections were deparaffinized, exposed

(9) Exosomes Derived from Yak Follicular Fluid Increase 2-Hydroxyestradiol Secretion by Activating Autophagy in Cumulus CellsAnimals : an open access journal from MDPINovember 16, 2022Xu R, Wang J, Wang M, Gao L, Zhang R, Zhao L, Liu B, Han X, Baloch AR, Cui Y, et al.with a secondary antibody (Goat Anti-Rabbit IgG-HRP) [23]. Bands were visualized using an ECL kit (AB65623, Abcam, Cambridge, UK) and detected using the Amersham Imager 600 system (GE Healthcare Life Sciences,

(10) Nuclear paraspeckle assembly transcript 1 promotes the podocyte injury via targeting miR-23b-3p/B-cell lymphoma-2 interacting protein 3 like axisRen FailNovember 9, 2022Jing Wang, Junpeng Luo, Li Du, Xin Shu, Chengyu Guo, Tanshi Li(TBST; ab64204; Abcam, Cambridge, UK), and incubated utilizing enhanced chemiluminescence (ECL) kit (ab65623; Abcam, Cambridge, UK) for visualizing. After the membrane was exposed under iBright™ CL1500 Imaging

(11) Bcl-xL activity influences outcome of the mitotic arrestFrontiers in PharmacologySeptember 15, 2022M. Suleimenov, S. Bekbayev, M. Ten, N. Suleimenova, M. Tlegenova, A. Nurmagambetova, S. Kauanova, I. Vorobjevgoat anti-rabbit IgG H&L HRP (ab6721, 1:5000, Abcam, United States) was used. Immunoblots were visualized with an ECL reagent (ab65623, Abcam, United States) using a Bio-Rad imaging system. Statistical analysis For comparison of two

(12) Design and Microinjection of Morpholino Antisense Oligonucleotides and mRNA into Zebrafish Embryos to Elucidate Specific Gene Function in Heart DevelopmentJournal of Visualized ExperimentsAugust 29, 2022Zain Zaki Zakaria, Shahram Eisa-Beygi, Fatiha M. Benslimane, Ramani Ramchandran, Huseyin C. Yalcincat. no C861C44, Electrochemiluminescence (ECL) kit,Abcam Biochemicals,Abcam Biochemicals cat. no ab65623, Glycine,Sigma,"Sigma, cat. no C988U91", Goat anti Rabbit,Abcam,Abcam- ab6721,Goat Anti-Rabbit IgG H&L (HRP

(13) Expression of Key Factors of the Hippo Signaling Pathway in Yak (Bos grunniens) Mammary GlandAnimals (Basel)August 17, 2022Peiyan Du, Bin Lu, Ling Zhao, Ying Yao, Wenjie Qian, Zhen Yu, Yan Cui, Sijiu Yu, Jiangfeng Fanwith PBS buffer three times (10 min, each). The membrane was then incubated with an ECL substrate kit (AB65623, Abcam, Cambridge, UK). In the negative control group, the primary antibody was replaced with 1:400

(14) Arctigenin inhibits apoptosis, extracellular matrix degradation, and inflammation in human nucleus pulposus cells by up‐regulating miR ‐483‐3pJournal of Clinical Laboratory AnalysisJuly 1, 2022Zhe Ji, Rui Guo, Zhigang Ma, Hongwei Liprotein bands were visualized by iBright CL750 (Thermo Fisher) with an ECL Western Blotting Substrate (ab65623, Abcam). The relative expressions of proteins were quantitated by ImageJ2x (Rawak Software). 2.6 Cell transfection

(15) Glabridin, a bioactive component of licorice, ameliorates diabetic nephropathy by regulating ferroptosis and the VEGF/Akt/ERK pathwaysMolecular medicine (Cambridge, Mass.)May 20, 2022Tan H, Chen J, Li Y, Li Y, Zhong Y, Li G, Liu L, Li Y.antibodies overnight, followed by incubated with secondary antibody for 2 h. Finally, by using an ECL kit (#ab65623; Abcam), the protein bands were visualized, of which intensities were measured by Image J 6.0 software.

(16) Emodin suppresses hepatocellular carcinoma growth by regulating macrophage polarization via microRNA-26a/transforming growth factor beta 1/protein kinase BBioengineeredApril 1, 2022Yin J, Zhao X, Chen X, Shen G.Goat Anti-Rabbit IgG H&L (HRP) (cat.ab6721). The protein bands were visualized using an ECL Western Blotting Substrate Kit (cat. ab65623, Abcam). In vivo assay The experimental processes involving animals were carried out as Zhao et al.

(17) Circular RNA hsa_circ_0077837 is upregulated in non-small cell lung cancer to downregulate phosphatase and tensin homolog through methylationBioengineeredMarch 5, 2022Dezhi Li, Hongying Lv, Huijiang Gao, Zizong Wang, Dongfei Wang, Kaihua Tian, Lin Lisecondary antibody (ab6721, Abcam). Signals were then developed using ECL Western blotting Substrate Kit (ab65623, Abcam). Data were normalized using QuantityOne software. Cell apoptosis assay HCC827 cells were cultured

(18) Circular RNA circHIPK3 is downregulated in diabetic cardiomyopathy and overexpression of circHIPK3 suppresses PTEN to protect cardiomyocytes from high glucose-induced cell apoptosisBioengineeredMarch 1, 2022Jun Jiang, Guannan Gao, Qiang Pan, Jing Liu, Yu Tian, Xiaoji Zhang(ab6721, Abcam, Hangzhou, China) was used to incubate the membranes at room temperature for 3 h. ECL (ab65623, Abcam, Hangzhou, China) was used for the production of signals. Quantity One software was used for

(19) METTL14 benefits the mesenchymal stem cells in patients with steroid-associated osteonecrosis of the femoral head by regulating the m6A level of PTPN6Aging (Albany NY)December 31, 2021Cheng Cheng, Haoping Zhang, Jia Zheng, Yi Jin, Donghui Wang, Zhipeng Daiantibody was used to develop the blots. Blots were detected with the ECL Western Blotting Substrate Kit (ab65623, Abcam) on an ECL system (Bio-Rad, USA). m6A RNA methylation quantification The content of m6A in

(20) Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa CellsCurrent Issues in Molecular BiologyDecember 10, 2021Truong Xuan Dai et al.washed three times with 1X TBST. The blots were visualized using a ECL Western Blotting Substrate Kit (ab65623, Abcam, Cambridge, MA, USA). Imaging was carried out with an X-ray film in a darkroom. 2.7. Microfilament

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ECL Western Blotting Substrate Kit from Abcam

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ECL Western Blotting Substrate Kit from Abcam

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